منابع مشابه
Toward the Proteome of the Human Peripheral Blood Eosinophil.
Eosinophils are granular leukocytes that have significant roles in many inflammatory and immunoregulatory responses, especially asthma and allergic diseases. We have undertaken a fairly comprehensive proteomic analysis of purified peripheral blood eosinophils from normal human donors primarily employing 2-dimensional gel electrophoresis with protein spot identification by matrix-assisted laser ...
متن کاملEosinophil purification from peripheral blood.
Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1-4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by nega...
متن کاملPeripheral blood eosinophil counts and bronchial responsiveness.
Bronchial responsiveness (histamine PC20) and peripheral blood eosinophil counts were measured in 23 asthmatic subjects, of whom 14 were atopic and nine non-atopic. In the group as a whole there was an inverse correlation between baseline eosinophil count and histamine PC20 (r = -0.71; p less than 0.001). For atopic subjects a relationship between eosinophil count and histamine PC20 was observe...
متن کاملHeterogeneity of Human Peripheral Blood Eosinophil - Type Colonies :
We have recently shown that a proportion of previously designated human eosinophil “(Eo)-type” colonies in methylcellulose contain basophils and histamine (Denburg et al Blood 61 :775, 1 983). In the present studies. individual Eo-type colonies have been analyzed by cell morphology as well as by biochemical assays for histamine, CharcotLeyden crystal protein (dC). and eosinophil granule major b...
متن کاملHeterogeneity of human peripheral blood eosinophil-type colonies: evidence for a common basophil-eosinophil progenitor.
We have recently shown that a proportion of previously designated human eosinophil "(Eo)-type" colonies in methylcellulose contain basophils and histamine (Denburg et al Blood 61:775, 1983). In the present studies, individual Eo-type colonies have been analyzed by cell morphology as well as by biochemical assays for histamine, Charcot-Leyden crystal protein (CLC), and eosinophil granule major b...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2016
ISSN: 1535-3893,1535-3907
DOI: 10.1021/acs.jproteome.6b00006